Characterization of ORF127 of Helicoverpa armigera nucleopolyhedrovirus
نویسندگان
چکیده
منابع مشابه
Functional analysis of FP25K of Helicoverpa armigera single nucleocapsid nucleopolyhedrovirus.
The fp25k gene of Helicoverpa armigera single nucleocapsid nucleopolyhedrovirus (HearNPV) was studied. HearNPV fp25k gene transcription was found starting from about 18 h post-infection, and protein could be detected from the same time with antiserum against FP25K. To study the function of HearNPV fp25k, a recombinant HearNPV (HaBacWD11) with an enhanced green fluorescent protein (GFP) gene rep...
متن کاملThe sequence of the Helicoverpa armigera single nucleocapsid nucleopolyhedrovirus genome.
The nucleotide sequence of the Helicoverpa armigera single-nucleocapsid nucleopolyhedrovirus (HaSNPV) DNA genome was determined and analysed. The circular genome encompasses 131,403 bp, has a G+C content of 39.1 mol% and contains five homologous regions with a unique pattern of repeats. Computer-assisted analysis revealed 135 putative ORFs of 150 nt or larger; 100 ORFs have homologues in Autogr...
متن کاملCharacterization of ORF2 and its encoded protein of the Helicoverpa armigera nucleopolyhedrovirus.
The open reading frame 2 (ha2) of the Helicoverpa armigera single nucleocapsid nucleopolyhedrovirus (HaSNPV), a conserved gene in most baculoviruses from lepidopteran insects such as p78/83 of the Autographa californica MNPV, was characterized. It is 1,242 bp long and potentially encodes a 45.9 kDa. Ha2 is conserved among baculoviruses from lepidopteran insects. Ha2 transcripts were detected fr...
متن کاملMolecular and biological characteristics of some geographic isolates of nucleopolyhedrovirus of Helicoverpa armigera (Lep.: Noctuidae)
هفت جدایه از ویروس Helicoverpa armigera NPV با تکنیک PCR-RFLP توصیف گردید و بیماری‌زایی نسبی آنها نسبت به لاروهای سن دوم H. armigera (Hübner) قبل و بعد از پرتوتابی با شبه نور خورشید و شرایط محیط آزاد روی دو گیاه پنبه و گوجه‌فرنگی مورد مقایسه قرار گرفت. محصول PCR حاصل از ژن 25K FP تفاوتی بین جدایه‌ها نشان نداد (780 جفت باز). محصول پلیمراز به دست آمده از جدایه‌ها پس از آنالیز جداگانه با چهار نوع ...
متن کاملCharacterization of the Helicoverpa Armigera Nucleopolyhedrovirus During Serial Passage in Cell Culture
Helicoverpa armigera nucleopolyhedrovirus (HearNPV) is highly pathogenic to the cotton bollworm, H. armigera. There have been several attempts to develop in vitro production of this virus, including the selection of high producing cell line. In order to evaluate the potential of in vitro methods of producing this virus on a large-scale, the virus was propagated in the H. armigera (HAPO2) and H....
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ژورنال
عنوان ژورنال: Acta Virologica
سال: 2009
ISSN: 1336-2305
DOI: 10.4149/av_2009_04_247